For any complaints and enquiries you may contact us here.If we are not able to resolve a complaint, you may submit it through the EU online dispute resolution platform at The Direct RNA Sequencing kit is new and both accuracy and throughput will continue to improve with coming kit versions.
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Following first-strand synthesis, reverse transcriptases tend to add 1–3 non-templated Cs to the 3’ end of the c DNA strand, and by including an additional primer in the reaction which anneals to the non- templated Cs, we can make the RT enzyme switch templates, to extend opposite this primer. There are several ways to prepare RNA strands for sequencing. 1 shows one method, where a c DNA strand is synthesised, but not sequenced. This allows efficient threading of the RNA strand into the nanopore, and is pre-loaded with the motor protein which controls the speed of translocation of the strand.